FungiFun3 is an updated web-based gene set enrichment tool specifically designed for fungal omics data. It now supports over 1,200 fungal strains—more than four times its previous version—and includes ranked gene set enrichment analysis. With a modernized interface, it enables the analysis of a wide range of fungi, including WHO-listed human pathogens. Available freely at: https://fungifun3.hki-jena.de.